June 21, 2017

En Huang, PhD

En Huang
Assistant Professor
 

Education

2013, PhD, The Ohio State University, Food Microbiology
2009, MS, Southern Illinois University Carbondale, Plant and Soil Science
2007, MS, South China University of Technology, Fermentation Engineering
2004, BS, Southwest University, China, Food Science

Research Interests

The main theme of my research is to develop novel antimicrobial agents which have the potential to be used as natural food preservatives, animal feed additives or novel antibiotics against drug-resistant bacterial pathogens. We use natural antimicrobials such as bacteriophage, bacteriocin, and essential oil compounds to enhance food safety. My other research primarily focuses on developing novel peptide and lipopeptides as new drug candidates for the treatment of bacterial infections caused by antibiotic-resistant bacterial pathogens. I contributed to the discovery and development of several novel antimicrobial agents, particularly paenibacterin (lipopeptide) and paenipeptin (lipopeptide). These novel antimicrobial compounds show potent activity against antibiotic-resistant pathogens. After the discovery of these antimicrobials, we determined their mechanisms of action, toxicity, biosynthesis, and therapeutic efficacy in animal models.

Areas of Expertise

  • Food Microbiology and Safety
  • New antimicrobial drug discovery

Courses Taught

  • Environmental Biological Hazards (ENVH 5404)
  • Environmental Exposure Assessment (ENVH 5222)

Publications

Moon S. H. and Huang E. 2018. Lipopeptide paenipeptin analogues potentiate clarithromycin and rifampicin against carbapenem-resistant pathogens. Antimicrobial agents and Chemotherapy. 62: e00329-18

Moon S. H., Zhang X., Zheng G., Meeker D. G., Smeltzer M. S., and Huang E. 2017. Novel linear lipopeptide paenipeptins with potential for eradicating biofilms and sensitizing Gram-negative bacteria to rifampicin and clarithromycin. Journal of Medicinal Chemistry. 60, 9630-9640.

Huang E., Yang X., Zhang L., Moon S.H., and Yousef A.E. 2017. New Paenibacillus strain produces a family of linear and cyclic antimicrobial lipopeptides: cyclization is not essential for their antimicrobial activity. FEMS Microbiology Letters, 364, fnx049.

Yesil M., Huang E., Yang X., and Yousef A. E. 2017. Complete genome sequence of Escherichia phage OSYSP. Genome announcements, 5(42), e00880-17.

Yang X., Huang E., and Yousef A.E. 2017. Brevibacillin, a cationic lipopeptide that binds to lipoteichoic acid and subsequently disrupts cytoplasmic membrane of Staphylococcus aureus. Microbiological Research, 195, 18-23.

Patents

  • Yousef A.E., Guo, Y., Huang E., Yuan C. and Zhang L. 2011. Antimicrobial agent, bacterial strain, biosynthesis, and methods of use. (Patent number: US 9,017,692 B2)
  • Yousef A.E., Guo, Y., and Huang E. 2013. Biosynthesis of paenibacillin. (Publication number: WO/2013/180699)

Professional Activities

Professional Service
Associate Editor: Microbiological Research

Ad-hoc reviewer for the following Journals:

  • Applied Microbiology and Biotechnology
  • Enzyme and Microbial Technology
  • Food and Bioprocess Technology
  • Food Control
  • Foodborne Pathogens and Disease
  • Journal of Food Safety,
  • Meat Science
  • Microbial Pathogenesis

Professional Affiliations

  • American Society of Microbiology, 2012-present
  • Institute of Food Technologists, 2010-present
  • International Association of Food Protection, 2011-present