June 21, 2017

En Huang, PhD

Assistant Professor


2013  – Ph.D., Food Microbiology, The Ohio State University
2009 – M.S., Plant and Soil Science, Southern Illinois University Carbondale
2007 – M.S., Fermentation Engineering, South China University of Technology
2004 – B.S., Food Science, Southwest University, China

Research Interests

The main theme of my research is to develop natural antimicrobial compounds to enhance the safety and quality of food. One of my research focuses is to discover and characterize natural antimicrobial peptides (e.g., bacteriocins) and lipopeptides using chemical and genetic tools. These antimicrobial agents have the potential to be used as natural food preservatives, animal feed additives or novel antibiotics against drug-resistant bacterial pathogens. My other research interest lies in studying the transmission, ecology and evolution of foodborne pathogens and spoilage microorganisms using genomic, transcriptomic, and metagenomic approaches.

Areas of Expertise

  • Bacteriocins
  • Lipopeptides
  • Fermentations
  • Food Safety
  • Food Microbiology
  • Foodborne pathogens


Yang X., Huang E., Yuan C., Zhang L., and Yousef A.E. 2016. Isolation and structural elucidation of Brevibacillin, an antimicrobial lipopeptide from Brevibacillus laterosporus that combats drug-resistant Gram-positive bacteria. Applied and Environmental Microbiology 82, 2763-2772

Guo Y., Huang E., Yang X., Zhang L., Yousef A.E., Zhong J. 2016. Isolation and characterization of a Bacillus atrophaeus strain and its potential use in food preservation. Food Control, 60, 511-518.

Huang E. and Yousef A.E. 2015. Biosynthesis of paenibacillin, a lantibiotic with N-terminal acetylation, by Paenibacillus polymyxa. Microbiological Research. 181, 15-21.

Gerst M.M., Huang E., Zhang L., and Yousef A.E. 2015. Development of a new paenibacillin-producing strain and testing its usability in improving food safety. Journal of Food Science, 80, M1538-M1543.

Huang E. and Yousef A.E. 2014. Lipopeptide antibiotic, paenibacterin, binds to bacterial outer membrane and exerts bactericidal activity through cytoplasmic membrane damage. Applied and Environmental Microbiology, 80, 2700-2704.

Huang E. and Yousef A.E. 2014. Paenibacterin, a Novel broad-spectrum lipopeptide antibiotic, neutralizes endotoxins and promotes survival in a murine model of Pseudomonas aeruginosa-induced sepsis. International Journal of Antimicrobial Agents, 44: 74-77.

Huang E., Guo Y. and Yousef A.E. 2014. Biosynthesis of the new broad-spectrum lipopeptide antibiotic paenibacterin in Paenibacillus thiaminolyticus OSY-SE.  Research in Microbiology, 165, 243-251.

Huang E., Zhang L., Chung Y.C., Zheng Z, and Yousef A.E. 2013. Characterization and application of enterocin RM6, a bacteriocin from Enterococcus faecalis. Biomed Research International, vol. 2013, Article ID 206917.

Huang E., Guo Y., and Yousef, A.E. 2012. Draft genome sequence of Paenibacillus sp. OSY-SE, a bacterium produces a novel broad-spectrum lipopeptide antibiotic, paenibacterin. Journal of Bacteriology, 194: 6306-6306.

Guo Y., Huang E., Yuan C., Zhang L., and Yousef A.E. 2012. Isolation of a Paenibacillus sp. strain and structural elucidation of its broad-spectrum lipopeptide antibiotic. Applied and Environmental Microbiology, 78: 3156-3165.

Huang E. and Yousef A.E. 2012. Draft genome sequence of Paenibacillus polymyxa OSY-DF that coproduces a lantibiotic, paenibacillin, and polymyxin E1. Journal of Bacteriology, 194: 4739-4740.


Yousef A.E., Guo, Y., Huang E., Yuan C. and Zhang L. 2011. Antimicrobial agent, bacterial strain, biosynthesis, and methods of use. (Patent number: US 9,017,692 B2)

Yousef A.E., Guo, Y., and Huang E. 2013. Biosynthesis of paenibacillin. (Publication number: WO/2013/180699)

Professional Activities

Professional Service

Associate Editor: Microbiological Research

Ad-hoc reviewer for the following Journals:
Applied Microbiology and Biotechnology, Enzyme and Microbial Technology, Food and Bioprocess Technology, Food Control, Foodborne Pathogens and Disease, Journal of Food Safety, Meat Science, Microbial Pathogenesis

Professional Affiliations

American Society of Microbiology, 2012-present

Institute of Food Technologists, 2010-present

International Association of Food Protection, 2011-present