June 21, 2017

En Huang, PhD

Assistant Professor
 

Education

2013, PhD, The Ohio State University, Food Microbiology
2009, MS, Southern Illinois University Carbondale, Plant and Soil Science
2007, MS, South China University of Technology, Fermentation Engineering
2004, BS, Southwest University, China, Food Science

Research Interests

The main theme of my research is to develop novel antimicrobial agents which have the potential to be used as natural food preservatives, animal feed additives or novel antibiotics against drug-resistant bacterial pathogens. We use natural antimicrobials such as bacteriophage, bacteriocin, and essential oil compounds to enhance food safety. My other research primarily focuses on developing novel peptide and lipopeptides as new drug candidates for the treatment of bacterial infections caused by antibiotic-resistant bacterial pathogens. I contributed to the discovery and development of several novel antimicrobial agents, particularly paenibacterin (lipopeptide) and paenipeptin (lipopeptide). These novel antimicrobial compounds show potent activity against antibiotic-resistant pathogens. After the discovery of these antimicrobials, we determined their mechanisms of action, toxicity, biosynthesis, and therapeutic efficacy in animal models.

Areas of Expertise

  • Food Microbiology and Safety
  • New antimicrobial drug discovery

Courses Taught

  • ENVH 5404 – Environmental Biological Hazards
  • ENVH 5222 – Environmental Exposure Assessment

Publications

Moon S.H., Udaondo Z., Li X., Yang X., Jun S.R., Huang E. 2021. Isolation and characterization of carbapenemase-producing and polymyxin B-resistant Enterobacter bugandensis from a vegetable. Journal of Global Antimicrobial Resistance, 26, 264-265. https://doi.org/10.1016/j.jgar.2021.07.013

Huang E., Hussein W.E., Campbell E.P., Yousef A.E. 2021. Applications in food technology: Antimicrobial Peptides. In Wu J. & Toldrá F. (Eds.) Biologically Active Peptides. Academic Press (Elsevier). https://doi.org/10.1016/B978-0-12-821389-6.00006-6

Moon S.H., Kaufmann Y., Huang E. 2020. Paenipeptin analogues potentiate clarithromycin and rifampicin against mcr-1 mediated polymyxin-resistant Escherichia coli in vivo. Antimicrobial Agents and Chemotherapy, 64 (4), e02045-19. https://doi.org/10.1128/AAC.02045-19

Moon S.H., Waite-Cusic J., Huang E. 2020. Control of Salmonella in chicken meat using a combination of a commercial bacteriophage and plant-based essential oils. Food Control, 110, 106984. https://doi.org/10.1016/j.foodcont.2019.106984

Moon S.H. and Huang E. 2019. Novel linear lipopeptide paenipeptin C′ binds to lipopolysaccharides and lipoteichoic acid and exerts bactericidal activity by the disruption of cytoplasmic membrane. BMC Microbiology, 19(1), 6. https://doi.org/10.1186/s12866-018-1381-7

https://www.ncbi.nlm.nih.gov/myncbi/en.huang.2/bibliography/public/

Grants

Novel linear lipopeptide paenipeptins sensitize multidrug-resistant Gram-negative pathogens to antibiotics. National Institute of Allergy and Infectious Diseases (NIAID)/NIH. 7/1/2019-1/31/2022, R21AI146693. Role: PI

Novel linear lipopeptides targeting antibiotic-resistant Gram-negative pathogens. COBRE: Center for Microbial Pathogenesis and Host Inflammatory Responses (Mark Smeltzer, Director). National Institute of General Medical Sciences (NIGMS)/NIH, P20 GM103625. 5/1/2018-6/30/2019. Role: Pilot Project Leader.

Patents

Huang E, Moon SH, Smeltzer M, and Meeker D. 2018. Linear polypeptide paenipeptins and methods of using the same. PCT Patent Application No. PCT/US2018/45395

Yousef AE, Guo Y, Huang E, Yuan C, and Zhang L. 2011. Antimicrobial agent, bacterial strain, biosynthesis, and methods of use. Patent number: US 9,017,692 B2

Professional Activities

Professional Service
Associate Editor: Microbiological Research

Editorial board: Food Research International

Professional Affiliations

  • American Society for Microbiology, 2012-present
  • Institute of Food Technologists, 2010-present
  • International Association for Food Protection, 2011-present